Multiplex PCR assay for identification of human diarrheagenic Escherichia coli.

نویسندگان

  • Claudia Toma
  • Yan Lu
  • Naomi Higa
  • Noboru Nakasone
  • Isabel Chinen
  • Ariela Baschkier
  • Marta Rivas
  • Masaaki Iwanaga
چکیده

A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregative E. coli. This assay allowed the categorization of a diarrheagenic E. coli strain in a single reaction tube.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 41 6  شماره 

صفحات  -

تاریخ انتشار 2003